Production and characterisation of human recombinant single chain urokinase-type plasminogen activator from mouse cells
Academic Article
Publication Date:
1989
Short description:
Production and characterisation of human recombinant single chain urokinase-type plasminogen activator from mouse cells / Nolli, M.L., Sarubbi, E., Corti, A., Robbiati, F., Soflientini, A., Blasi, F., Parenti, F., Cassani, G.. - In: FIBRINOLYSIS. - ISSN 0268-9499. - 3:2(1989), pp. 101-106. [10.1016/0268-9499(89)90038-6]
abstract:
Human chromosomal DNA coding for single chain urokinase-type plasminogen activator (scu-PA) has been inserted into a vector and expressed in LB6 cells. The recombinant clone termed 1C9 is stable and produces scu-PA at about 0.8 mg/I/day in a bioreactor. The scu-PA produced is human by amino- and carboxy-terminal analysis and immunoblotting. The recombinant scu-PA is glycosylated and fully activatable by plasmin. © 1989.
Iris type:
1.1 Articolo in rivista
Keywords:
Single chain urokinase-type plasminogen activator
List of contributors:
Nolli, M. L.; Sarubbi, E.; Corti, A.; Robbiati, F.; Soflientini, A.; Blasi, F.; Parenti, F.; Cassani, G.
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