Publication Date:
2010
abstract:
OBJECTIVE
Evaluate the occurrence of cytotoxic effects induced by cocaine on mammalian cells in vitro considering that habitual insufflation of cocaine may cause extensive destruction of the osteocartilaginous structures of the nose, sinuses and palate, though its pathogenesis is still unknown.
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METHOD
We measured the effect of cocaine in vitro on human epithelial cells (HaCat cells) at different concentrations and times of exposure in 2 groups (case and control). HaCat cells were incubated with 0.1 μM, 1μM, and 10μM of cocaine for 5, 24 and 48 hours. Cells were fixed and studied with immunofluorescence.
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RESULTS
We found a high density of apoptotic events and aberrant mitoses such as abnormal mitotic spindles with multiple poles compared to the control group in a time and dose dependent way.
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CONCLUSION
Cocaine exposure of mammalian cells in vitro can induce dose- and time-dependent apoptosis with anomalies in mitotic spindle formation, possibly associated with abnormalities in the number and functioning of centrosomes.
Evaluate the occurrence of cytotoxic effects induced by cocaine on mammalian cells in vitro considering that habitual insufflation of cocaine may cause extensive destruction of the osteocartilaginous structures of the nose, sinuses and palate, though its pathogenesis is still unknown.
Previous Section
Next Section
METHOD
We measured the effect of cocaine in vitro on human epithelial cells (HaCat cells) at different concentrations and times of exposure in 2 groups (case and control). HaCat cells were incubated with 0.1 μM, 1μM, and 10μM of cocaine for 5, 24 and 48 hours. Cells were fixed and studied with immunofluorescence.
Previous Section
Next Section
RESULTS
We found a high density of apoptotic events and aberrant mitoses such as abnormal mitotic spindles with multiple poles compared to the control group in a time and dose dependent way.
Previous Section
CONCLUSION
Cocaine exposure of mammalian cells in vitro can induce dose- and time-dependent apoptosis with anomalies in mitotic spindle formation, possibly associated with abnormalities in the number and functioning of centrosomes.
Iris type:
1.5 Abstract in rivista
List of contributors:
Trimarchi, Matteo; Bellini, C; Limardo, P; Bussi, M.
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