INFLUANCE OF NOVEL NANOTITANIUM IMPLANT SURFACE ON HUMAN OSTEOB

Purpose: The aim qfrhC' srudy is to im•esrigate human osreohlast-lfke celi hd1cn•ior and grmrth in the prcsence of 3 differenl titanium unplam .WT.frxes. Mate1ials: Hwnan sre111 cells 11'ere .firsr obrained and then sorted hy fluorescence-actimred ce!! sm1er .from mesench.\7nal stem celi clmters o.f lw­ man denral papi/la. The obtained hwnon dentol papi/la stem cells ll'ere induced m differentiate imo osreohlosr-/ike ce/ls ond were rhen anoly::.ed /;y re1•erse rran­ scriptase po/ymerose choi11 reocro11 and Wesrem b/or analyses. The ceils pm­ liferared ond were culrured omo 3 d(f­ .ferent ritanium discs ( sandhlasred. sondblosted o11d lorge-grir acid­ erched. ond full comacr cm•erage [FCCj) and anoi,Y:ed by scanning elecrron micmscope. ing the healing period.'' An essential role of osteogenesis processes is played by os­ lcoblast progenitor sLem cells during recmitment. adhesion. proliferation. differ­ entimion. and mmeralized mauix deposi­ tion dllling bone regenera.Lion phases.111 1 Aduh human denral pulp ancl young: hu­ man dcntal papilb culturcd in osteogenic medium shO\\ cellular phenotypes and minera.hzing capabiliLies sm1il u• to Lhose of osLeoblasL .1'• ' Numerous bone regen­ eraLive sludics- :" have foundecl lÌlat hu­ man dcntal papilla slcm cdls (hDPaSCsl. similar l0 Jdull sten celi!->.can difk:•cmiale imo speciali:::.ed c.:cllty!1es b0th in ,.:,.r, and in l'i/i';l .;nu:UJOJlS. C,1\i[Ìnning the f lO.Ìbi\­ Ìl\ 0f the:< .: Il lin :; 'l-'flr• CXj'l:.:;irnenlal Results: In ali cmalyses .wmplcs. a high ce/l actil•iry 11'0.\' obsenwl. 1rith G'pical osteoh/ast mawre mor­ phosrructural respon.1•e 011 rough sw.face. 17le high nwnber c!f•osteoblast­ like cel/s was.found 011 ritcmiwn FCC discs. At the .mme time. scamzing electron microscnpe analysis co11- .firmed the high hiocomparihili0• (!( this swface. Conclusion: T11e rapid manrration of the osteoblast-like cells nn FCC rira- 11Ìwn sw.face suggests thar rhis stntcwre cnuld play a cenrral mie during i11irial plwses o.f bone heali11g processes.