Transcriptional dynamics of induced pluripotent stem cell differentiation into β cells reveals full endodermal commitment and homology with human islets
Academic Article
Publication Date:
2021
abstract:
Induced pluripotent stem cells (iPSCs) have the capacity to generate β cells in vitro, but the differentiation is incomplete and generates a variable percentage of off-target cells. Single-cell RNA sequencing offers the possibility of characterizing the transcriptional dynamics throughout differentiation and determining the identity of the final differentiation product.
Iris type:
1.1 Articolo in rivista
Keywords:
endocrine cells; induced pluripotent stem cells; pseudotime; single-cell RNA sequencing; β-cell differentiation
List of contributors:
Pellegrini, Silvia; Chimienti, Raniero; Scotti, Giulia Maria; Giannese, Francesca; Lazarevic, Dejan; Manenti, Fabio; Poggi, Gaia; Lombardo, Marta Tiffany; Cospito, Alessandro; Nano, Rita; Piemonti, Lorenzo; Sordi, Valeria
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