Data di Pubblicazione:
2009
Citazione:
Early activation of fibrogenesis in transplanted kidneys: A study on serial renal biopsies / DEL PRETE, D., Ceol, M., Anglani, F., Vianello, D., Tiralongo, E., Valente, M., Graziotto, R., Bonfante, L., Scaparrotta, G., Furian, L., Rigotti, P., Gambaro, G., D'Angelo, A.. - In: EXPERIMENTAL AND MOLECULAR PATHOLOGY. - ISSN 0014-4800. - 87:2(2009), pp. 141-145. [10.1016/j.yexmp.2009.07.004]
Abstract:
Abstract
BACKGROUND/AIMS: In kidney transplants, the renin-angiotensin system (RAS) is involved in systemic and local changes that may induce fibrosis. Our aim was to use gene expression and immunohistochemical analysis to investigate the RAS and several factors involved in the fibrogenic cascade in allograft biopsies.
METHODS: We considered 43 donor biopsies (T0), 18 biopsies obtained for diagnostic purposes (Td) and 24 protocol biopsies (Tp) taken 2 months after transplantation in patients with stable renal function. Morphometric alpha SMA and TGF beta 1 analysis, and Masson's Trichrome staining were performed. mRNA levels of angiotensinogen, renin, ACE, AT1-R, AT2-R, TGF beta 1, BMP-7, Coll III, fibronectin and alpha SMA were analyzed by real-time RT/PCR. MDRD a year after the transplant was also considered.
RESULTS: Significantly higher levels of AT1-R and alpha SMA transcripts were found in Tp than in T0. Regression analysis showed significant TGF beta 1-independent positive correlations between RAS and matrix components in T0 and Tp, but more evident in Tp, where a positive correlation between TGF beta 1 and Masson's Trichrome stained areas was also seen.
CONCLUSION: Our results suggest that RAS and TGF beta 1-related fibrogenic loops are activated as early as 2 months after kidney transplantation.
BACKGROUND/AIMS: In kidney transplants, the renin-angiotensin system (RAS) is involved in systemic and local changes that may induce fibrosis. Our aim was to use gene expression and immunohistochemical analysis to investigate the RAS and several factors involved in the fibrogenic cascade in allograft biopsies.
METHODS: We considered 43 donor biopsies (T0), 18 biopsies obtained for diagnostic purposes (Td) and 24 protocol biopsies (Tp) taken 2 months after transplantation in patients with stable renal function. Morphometric alpha SMA and TGF beta 1 analysis, and Masson's Trichrome staining were performed. mRNA levels of angiotensinogen, renin, ACE, AT1-R, AT2-R, TGF beta 1, BMP-7, Coll III, fibronectin and alpha SMA were analyzed by real-time RT/PCR. MDRD a year after the transplant was also considered.
RESULTS: Significantly higher levels of AT1-R and alpha SMA transcripts were found in Tp than in T0. Regression analysis showed significant TGF beta 1-independent positive correlations between RAS and matrix components in T0 and Tp, but more evident in Tp, where a positive correlation between TGF beta 1 and Masson's Trichrome stained areas was also seen.
CONCLUSION: Our results suggest that RAS and TGF beta 1-related fibrogenic loops are activated as early as 2 months after kidney transplantation.
Tipologia CRIS:
1.1 Articolo in rivista
Keywords:
Fibrosis; Gene expression; Immunohistochemistry; RAS; Renal biopsies; TGFβ1; Transplant; Adult; Biopsy; Fibrosis; Gene Expression; Humans; Immunohistochemistry; Kidney; Kidney Transplantation; Middle Aged; RNA; Messenger; Renin-Angiotensin System; Reverse Transcriptase Polymerase Chain Reaction; Transforming Growth Factor beta1; Gene Expression Profiling; Clinical Biochemistry; Molecular Biology; 2734
Elenco autori:
DEL PRETE, Dorella; Ceol, Monica; Anglani, Franca; Vianello, Daniela; Tiralongo, Emilia; Valente, Marialuisa; Graziotto, Romina; Bonfante, Luciana; Scaparrotta, Giuseppe; Furian, Lucrezia; Rigotti, Paolo; Gambaro, Giovanni; D'Angelo, Angela
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