BIOSYNTHESIS AND APICAL LOCALIZATION OF THE UROKINASE RECEPTOR IN POLARIZED MDCK EPITHELIAL-CELLS

The biosynthesis and the surface localization of the urokinase plasminogen activator receptor (uPaR) were analysed in MDCK epithelial cells and in unpolarized fibroblasts. No differences were observed with respect to rate of synthesis, nature of precursors and time of surface appearance. uPAR,vas localized particularly at the focal and cell-cell contacts when expressed in fibroblasts. On the contrary, in MDCK cells uPAR was found mostly on the apical surface; in agreement with its localization, down-regulation of uPAR by the uPA-PAI-1 complex was observed only from the apical membrane.