Data di Pubblicazione:
2010
Abstract:
PURPOSE:
The aim of the study is to investigate human osteoblast-like cell behavior and growth in the presence of 3 different titanium implant surfaces.
MATERIALS:
Human stem cells were first obtained and then sorted by fluorescence-activated cell sorter from mesenchymal stem cell clusters of human dental papilla. The obtained human dental papilla stem cells were induced to differentiate into osteoblast-like cells and were then analyzed by reverse transcriptase polymerase chain reaction and Western blot analyses. The cells proliferated and were cultured onto 3 different titanium discs (sandblasted, sandblasted and large-grit acid-etched, and full contact coverage [FCC]) and analyzed by scanning electron microscope.
RESULTS:
In all analyses samples, a high cell activity was observed, with typical osteoblast mature morphostructural response on rough surface. The high number of osteoblast-like cells was found on titanium FCC discs. At the same time, scanning electron microscope analysis confirmed the high biocompatibility of this surface.
CONCLUSION:
The rapid maturation of the osteoblast-like cells on FCC titanium surface suggests that this structure could play a central role during initial phases of bone healing processes.
The aim of the study is to investigate human osteoblast-like cell behavior and growth in the presence of 3 different titanium implant surfaces.
MATERIALS:
Human stem cells were first obtained and then sorted by fluorescence-activated cell sorter from mesenchymal stem cell clusters of human dental papilla. The obtained human dental papilla stem cells were induced to differentiate into osteoblast-like cells and were then analyzed by reverse transcriptase polymerase chain reaction and Western blot analyses. The cells proliferated and were cultured onto 3 different titanium discs (sandblasted, sandblasted and large-grit acid-etched, and full contact coverage [FCC]) and analyzed by scanning electron microscope.
RESULTS:
In all analyses samples, a high cell activity was observed, with typical osteoblast mature morphostructural response on rough surface. The high number of osteoblast-like cells was found on titanium FCC discs. At the same time, scanning electron microscope analysis confirmed the high biocompatibility of this surface.
CONCLUSION:
The rapid maturation of the osteoblast-like cells on FCC titanium surface suggests that this structure could play a central role during initial phases of bone healing processes.
Tipologia CRIS:
1.1 Articolo in rivista
Elenco autori:
Tetè, S; Mastrangelo, F; Quaresima, R; Vinci, Raffaele; Sammartino, G; Stuppia, L; Gherlone, FELICE ENRICO
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